RT Journal Article
SR Electronic
T1 Cytocompatibility of Bone Substitute Materials and Membranes
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 2035
OP 2040
DO 10.21873/invivo.12472
VO 35
IS 4
A1 SOGAND SCHAFER
A1 HAYDER AL-QADDO
A1 MARTIN GOSAU
A1 RALF SMEETS
A1 PHILIP HARTJEN
A1 REINHARD E. FRIEDRICH
A1 OLA A. NADA
A1 TOBIAS VOLLKOMMER
A1 ASHKAN RASHAD
YR 2021
UL http://iv.iiarjournals.org/content/35/4/2035.abstract
AB Background/Aim: With the demographic change and associated chronic bone loss, the need for cytocompatible bone replacement materials arise in modern medicine. The aim of this in vitro study was to investigate the cytocompatibility of eleven different bone substitute materials and membranes. Materials and Methods: Seven bone substitute materials and four membranes were assessed in vitro. The specimens were tested based on their interaction with MC3T3 pre-osteoblasts, through the utilization of viability, proliferation, and cytotoxicity assays. Cell vitality was evaluated using live-dead staining. Results: Although we found minor differences in cytocompatibility among the assessed materials, all tested materials can be considered as cytocompatible with a viability of more than 70% of the negative control, which indicates the non-toxic range as defined in current, international standards (DIN EN ISO 10993-5:2009, German Institute for Standardization, Berlin, Germany). Direct live-dead staining assays confirmed satisfactory cytocompatibility of all tested membranes. Conclusion: All examined bone substitute materials and membranes were found to be cytocompatible. In order to assess whether the observed minor differences can impact regenerative processes, further in vivo studies need to be conducted.