RT Journal Article
SR Electronic
T1 Detection of Novel Urine Markers Using Immune Complexome Analysis in Bladder Cancer Patients: A Preliminary Study
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 2073
OP 2080
DO 10.21873/invivo.12476
VO 35
IS 4
A1 NOZOMI AIBARA
A1 YASUYOSHI MIYATA
A1 KYOHEI ARAKI
A1 YUJI SAGARA
A1 KENSUKE MITSUNARI
A1 TOMOHIRO MATSUO
A1 KOJIRO OHBA
A1 YASUSHI MOCHIZUKI
A1 HIDEKI SAKAI
A1 KANAME OHYAMA
YR 2021
UL http://iv.iiarjournals.org/content/35/4/2073.abstract
AB Background/Aim: Little is known on urine biomarkers that are associated with malignant behavior in patients with bladder cancer (BC). Our aim was to identify BC-related factors in urine samples using our original method “immune complexome analysis”, based on detecting the immune complex (IC). Patients and Methods: Immune complexome analysis was performed using urine samples from 97 BC patients, including 67 with non-muscle invasive BC (NMIBC). Results: Eight IC-antigens were recognized as candidates for BC-related factors from 20,165 proteins. IC-serum albumin, -fibrinogen γ chain, -hemoglobin subunit α, -hemoglobin subunit β, -ceruloplasmin, and fibrinogen β chain were significantly associated with either pathological features and/or outcome. IC-ceruloplasmin was most widely associated with pathological features in all BC patients and lamina propria invasion and urinary tract recurrence in NMIBC. Conclusion: Based on detection of IC-antigens it was demonstrated that six IC-antigens, especially IC-ceruloplasmin, are potential urine biomarkers in BC.