PT  - JOURNAL ARTICLE
AU  - NOZOMI AIBARA
AU  - YASUYOSHI MIYATA
AU  - KYOHEI ARAKI
AU  - YUJI SAGARA
AU  - KENSUKE MITSUNARI
AU  - TOMOHIRO MATSUO
AU  - KOJIRO OHBA
AU  - YASUSHI MOCHIZUKI
AU  - HIDEKI SAKAI
AU  - KANAME OHYAMA
TI  - Detection of Novel Urine Markers Using Immune Complexome Analysis in Bladder Cancer Patients: A Preliminary Study
AID  - 10.21873/invivo.12476
DP  - 2021 Jul 01
TA  - In Vivo
PG  - 2073--2080
VI  - 35
IP  - 4
4099  - http://iv.iiarjournals.org/content/35/4/2073.short
4100  - http://iv.iiarjournals.org/content/35/4/2073.full
SO  - In Vivo2021 Jul 01; 35
AB  - Background/Aim: Little is known on urine biomarkers that are associated with malignant behavior in patients with bladder cancer (BC). Our aim was to identify BC-related factors in urine samples using our original method “immune complexome analysis”, based on detecting the immune complex (IC). Patients and Methods: Immune complexome analysis was performed using urine samples from 97 BC patients, including 67 with non-muscle invasive BC (NMIBC). Results: Eight IC-antigens were recognized as candidates for BC-related factors from 20,165 proteins. IC-serum albumin, -fibrinogen γ chain, -hemoglobin subunit α, -hemoglobin subunit β, -ceruloplasmin, and fibrinogen β chain were significantly associated with either pathological features and/or outcome. IC-ceruloplasmin was most widely associated with pathological features in all BC patients and lamina propria invasion and urinary tract recurrence in NMIBC. Conclusion: Based on detection of IC-antigens it was demonstrated that six IC-antigens, especially IC-ceruloplasmin, are potential urine biomarkers in BC.