PT  - JOURNAL ARTICLE
AU  - SEUNG-BO PARK
AU  - SEUNG HYO JUNG
AU  - HENGZHE JIN
AU  - SU JUNG KIM
AU  - YUNKYOUNG RYU
AU  - KYUNG-JIN LEE
AU  - BOKYUNG KIM
AU  - HYUN JIN SHIN
AU  - KYUNG-JONG WON
TI  - Bioluminescence Imaging of Matrix Metalloproteinases-2 and -9 Activities in Ethanol-injured Cornea of Mice
AID  - 10.21873/invivo.12405
DP  - 2021 May 01
TA  - In Vivo
PG  - 1521--1528
VI  - 35
IP  - 3
4099  - http://iv.iiarjournals.org/content/35/3/1521.short
4100  - http://iv.iiarjournals.org/content/35/3/1521.full
SO  - In Vivo2021 May 01; 35
AB  - Background/Aim: This study aimed to investigate the usefulness of in vivo bioluminescence imaging (BLI) to examine the role of matrix metalloproteinases (MMP)-2 and MMP-9 activation in the development and healing of ethanol-induced damage in the cornea of mice. Materials and Methods: Mouse corneal injury was induced by topical treatment with 20% ethanol. BLI was obtained from the ocular region of mice intravenously injected with an active-MMP-2/9 probe. In vivo results were validated in primary corneal epithelial cells. Results: BLI indicated that treatment of the eye with 20% ethanol elevated MMP-2/9 activity, which was inhibited by the application of eye drops (hyaluronic acid and serum). Treatment of corneal epithelial cells with 20% ethanol increased the activities of MMP-2 and MMP-9, which were also inhibited by eye drops. Conclusion: BLI can be applied in vivo in mice with corneal injury to examine the activity of MMPs and clarify the efficacy of eye drops.