TY - JOUR T1 - Bioluminescence Imaging of Matrix Metalloproteinases-2 and -9 Activities in Ethanol-injured Cornea of Mice JF - In Vivo JO - In Vivo SP - 1521 LP - 1528 DO - 10.21873/invivo.12405 VL - 35 IS - 3 AU - SEUNG-BO PARK AU - SEUNG HYO JUNG AU - HENGZHE JIN AU - SU JUNG KIM AU - YUNKYOUNG RYU AU - KYUNG-JIN LEE AU - BOKYUNG KIM AU - HYUN JIN SHIN AU - KYUNG-JONG WON Y1 - 2021/05/01 UR - http://iv.iiarjournals.org/content/35/3/1521.abstract N2 - Background/Aim: This study aimed to investigate the usefulness of in vivo bioluminescence imaging (BLI) to examine the role of matrix metalloproteinases (MMP)-2 and MMP-9 activation in the development and healing of ethanol-induced damage in the cornea of mice. Materials and Methods: Mouse corneal injury was induced by topical treatment with 20% ethanol. BLI was obtained from the ocular region of mice intravenously injected with an active-MMP-2/9 probe. In vivo results were validated in primary corneal epithelial cells. Results: BLI indicated that treatment of the eye with 20% ethanol elevated MMP-2/9 activity, which was inhibited by the application of eye drops (hyaluronic acid and serum). Treatment of corneal epithelial cells with 20% ethanol increased the activities of MMP-2 and MMP-9, which were also inhibited by eye drops. Conclusion: BLI can be applied in vivo in mice with corneal injury to examine the activity of MMPs and clarify the efficacy of eye drops. ER -