RT Journal Article
SR Electronic
T1 Detection of Circulating Gastrointestinal Cancer Cells in Conditionally Reprogrammed Cell Culture
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 1515
OP 1520
DO 10.21873/invivo.12404
VO 35
IS 3
A1 CHUN-SEOK YANG
A1 IN-HWAN KIM
A1 HYUN-DONG CHAE
A1 DAE-DONG KIM
A1 CHANG-HO JEON
YR 2021
UL http://iv.iiarjournals.org/content/35/3/1515.abstract
AB Background/Aim: The aim of this study was to detect circulating tumor cells (CTC) in the peripheral blood of gastrointestinal cancer patients using conditionally reprogrammed cell (CRC) culture. Materials and Methods: We confirmed the sensitivity of the CRC culture method. Five ml of blood were obtained from 81 cancer patients (56 colorectal and 25 gastric). The collected mononuclear cells were cultured for 4 weeks in the CRC condition. Finally, cultured cells were characterized by RT-PCR for the expression of hTERT and MAGE A1-6 mRNA. Results: The CRC method had a CTC detection limit of 6 cells for gastric cancer cells. After culture of 81 blood specimens, 38 formed visible cells, including 5 colonies. Among the 38 cells, 13 were hTERT positive and 4 were MAGE A1-6 positive. The final CTC detection rate was 16.0%. Conclusion: The CRC culture may potentially be used to evaluate the metastatic cancer cells in the circulation.