PT  - JOURNAL ARTICLE
AU  - CHUN-SEOK YANG
AU  - IN-HWAN KIM
AU  - HYUN-DONG CHAE
AU  - DAE-DONG KIM
AU  - CHANG-HO JEON
TI  - Detection of Circulating Gastrointestinal Cancer Cells in Conditionally Reprogrammed Cell Culture
AID  - 10.21873/invivo.12404
DP  - 2021 May 01
TA  - In Vivo
PG  - 1515--1520
VI  - 35
IP  - 3
4099  - http://iv.iiarjournals.org/content/35/3/1515.short
4100  - http://iv.iiarjournals.org/content/35/3/1515.full
SO  - In Vivo2021 May 01; 35
AB  - Background/Aim: The aim of this study was to detect circulating tumor cells (CTC) in the peripheral blood of gastrointestinal cancer patients using conditionally reprogrammed cell (CRC) culture. Materials and Methods: We confirmed the sensitivity of the CRC culture method. Five ml of blood were obtained from 81 cancer patients (56 colorectal and 25 gastric). The collected mononuclear cells were cultured for 4 weeks in the CRC condition. Finally, cultured cells were characterized by RT-PCR for the expression of hTERT and MAGE A1-6 mRNA. Results: The CRC method had a CTC detection limit of 6 cells for gastric cancer cells. After culture of 81 blood specimens, 38 formed visible cells, including 5 colonies. Among the 38 cells, 13 were hTERT positive and 4 were MAGE A1-6 positive. The final CTC detection rate was 16.0%. Conclusion: The CRC culture may potentially be used to evaluate the metastatic cancer cells in the circulation.