TY - JOUR T1 - Induction of Immunogenic Cell Death of Esophageal Squamous Cell Carcinoma by 5-Fluorouracil and Cisplatin JF - In Vivo JO - In Vivo SP - 743 LP - 752 DO - 10.21873/invivo.12315 VL - 35 IS - 2 AU - JUNYA NISHIMURA AU - SOTA DEGUCHI AU - HIROAKI TANAKA AU - YOSHIHITO YAMAKOSHI AU - MAMI YOSHII AU - TATSURO TAMURA AU - TAKAHIRO TOYOKAWA AU - SHIGERU LEE AU - KAZUYA MUGURUMA AU - MASAICHI OHIRA Y1 - 2021/03/01 UR - http://iv.iiarjournals.org/content/35/2/743.abstract N2 - Background/Aim: Neoadjuvant chemotherapy (NAC) using 5-FU (5-fluorouracil)/CDDP (cisplatin) is a standard therapy for stage II/III thoracic esophageal squamous cell carcinoma (ESCC) in Japan. The aim of this study was to investigate whether 5-FU/CDDP could induce immunogenic cell death in ESCC cell lines. Materials and Methods: Tumor samples for immunohistochemistry were obtained from 50 patients (mean age=63.1 years) with pathological stage 0-IVa ESCC who underwent NAC followed by surgery. Cell lines T.T and KYSE30 were used for the in vitro experiments. Results: The concentrations of HMGB1 were elevated in the cell line supernatants treated with 5-FU/CDDP. 5-FU/CDDP treated dendritic cells (DCs) showed a mature phenotype, and enhanced T cell proliferation capacity. In addition, mature DCs were observed in surgical specimens with a histological response after treatment with 5-FU/CDDP chemotherapy. Conclusion: 5-FU/CDDP could induce immunogenic cell death in the tumor microenvironment of ESCC. ER -