PT - JOURNAL ARTICLE AU - THIRA ROJASAWASTHIEN AU - TOMOHIKO SHIRAKAWA AU - AYAKO WASHIO AU - TOSHIYUKI TSUJISAWA AU - TAKUMA MATSUBARA AU - ASAKO INOUE AU - UMEO TAKAHAMA AU - KEISUKE NAKASHIMA AU - SHOICHIRO KOKABU TI - Vignacyanidin Polyphenols Isolated from Vigna Angularis Bean Promote Osteoblast Differentiation AID - 10.21873/invivo.12328 DP - 2021 Mar 01 TA - In Vivo PG - 883--888 VI - 35 IP - 2 4099 - http://iv.iiarjournals.org/content/35/2/883.short 4100 - http://iv.iiarjournals.org/content/35/2/883.full SO - In Vivo2021 Mar 01; 35 AB - Background/Aim: An effective bone regenerative method needs to be established for the dental field. To identify a novel osteogenic factor for bone regeneration, we examined the effect of vignacyanidin (VIG) on osteoblastogenesis. Materials and Methods: W20-17 cells, MC3T3-E1 cells, and primary cultured murine calvarial osteoblasts were used. Osteoblast differentiation was stimulated by β-glycerophosphate, ascorbic acid, or bone morphogenetic protein (BMP)-4. Adipogenesis was induced using dexamethasone, 3-isobutyl-1-methylxanthine, insulin, and rosiglitazone. Differentiation or proliferation markers were determined using western blotting and/or the quantitative reverse transcription polymerase chain reaction. Adipogenic cells were visualized by Oil Red O staining. Results: VIG treatment increased the expression of osteoblastic markers and alkaline phosphatase activity of osteoblast-lineage cells in a concentration-dependent manner. However, adipogenesis and cell proliferation were not affected by VIG. Conclusion: VIG treatment promoted osteoblast differentiation in osteoblast-lineage cells.