RT Journal Article
SR Electronic
T1 PolyIC Induces Retinoic Acid-inducible Gene-I and Melanoma Differentiation-associated Gene 5 and Modulates Inflammation in Podocytes
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 147
OP 153
DO 10.21873/invivo.12242
VO 35
IS 1
A1 NAKATA, MASAMICHI
A1 SHIMADA, MICHIKO
A1 NARITA-KINJO, IKUYO
A1 NAGAWA, DAIKI
A1 KITAYAMA, KAZUTAKA
A1 HAMADATE, MISATO
A1 MIURA, NAOTAKE
A1 NOZAKA, MASASHI
A1 KAWAMURA, YOSUKE
A1 FUJITA, TAKESHI
A1 MURAKAMI, REIICHI
A1 IMAIZUMI, TADAATSU
A1 NAKAMURA, NORIO
A1 TOMITA, HIROFUMI
YR 2021
UL http://iv.iiarjournals.org/content/35/1/147.abstract
AB Background/Aim: Viral infection often exacerbates proteinuria, which has been suggested to be due to antiviral responses of podocytes. We examined the effect of polyinosinic-polycytidylic acid (polyIC) on the expression of retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5) in differentiated human podocytes in culture. Materials and Methods: The podocytes were treated with 2 ng/ml to 500 μg/ml of polyIC for 3 to 36 h, and also transfected with siRNA against RIG-I and MDA5. F-actin staining was performed to assess actin reorganization. Results: PolyIC induced the expression of RIG-I and MDA5 in dose- and time-dependent manner, accompanied with interferon-β (IFN-β) and interleukin-6 (IL-6) up-regulation and actin reorganization. Temporal knockdown of RIG-I by siRNA decreased IFN-β expression, while MDA5 siRNA inhibited IFN-β and IL-6 expression. Actin reorganization was attenuated by RIG-I and MDA5 knockdown. Conclusion: RIG-I and MDA5 may play a role in the antiviral responses of podocytes.