PT  - JOURNAL ARTICLE
AU  - NAKATA, MASAMICHI
AU  - SHIMADA, MICHIKO
AU  - NARITA-KINJO, IKUYO
AU  - NAGAWA, DAIKI
AU  - KITAYAMA, KAZUTAKA
AU  - HAMADATE, MISATO
AU  - MIURA, NAOTAKE
AU  - NOZAKA, MASASHI
AU  - KAWAMURA, YOSUKE
AU  - FUJITA, TAKESHI
AU  - MURAKAMI, REIICHI
AU  - IMAIZUMI, TADAATSU
AU  - NAKAMURA, NORIO
AU  - TOMITA, HIROFUMI
TI  - PolyIC Induces Retinoic Acid-inducible Gene-I and Melanoma Differentiation-associated Gene 5 and Modulates Inflammation in Podocytes
AID  - 10.21873/invivo.12242
DP  - 2021 Jan 01
TA  - In Vivo
PG  - 147--153
VI  - 35
IP  - 1
4099  - http://iv.iiarjournals.org/content/35/1/147.short
4100  - http://iv.iiarjournals.org/content/35/1/147.full
SO  - In Vivo2021 Jan 01; 35
AB  - Background/Aim: Viral infection often exacerbates proteinuria, which has been suggested to be due to antiviral responses of podocytes. We examined the effect of polyinosinic-polycytidylic acid (polyIC) on the expression of retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5) in differentiated human podocytes in culture. Materials and Methods: The podocytes were treated with 2 ng/ml to 500 μg/ml of polyIC for 3 to 36 h, and also transfected with siRNA against RIG-I and MDA5. F-actin staining was performed to assess actin reorganization. Results: PolyIC induced the expression of RIG-I and MDA5 in dose- and time-dependent manner, accompanied with interferon-β (IFN-β) and interleukin-6 (IL-6) up-regulation and actin reorganization. Temporal knockdown of RIG-I by siRNA decreased IFN-β expression, while MDA5 siRNA inhibited IFN-β and IL-6 expression. Actin reorganization was attenuated by RIG-I and MDA5 knockdown. Conclusion: RIG-I and MDA5 may play a role in the antiviral responses of podocytes.