<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">NAKATA, MASAMICHI</style></author><author><style face="normal" font="default" size="100%">SHIMADA, MICHIKO</style></author><author><style face="normal" font="default" size="100%">NARITA-KINJO, IKUYO</style></author><author><style face="normal" font="default" size="100%">NAGAWA, DAIKI</style></author><author><style face="normal" font="default" size="100%">KITAYAMA, KAZUTAKA</style></author><author><style face="normal" font="default" size="100%">HAMADATE, MISATO</style></author><author><style face="normal" font="default" size="100%">MIURA, NAOTAKE</style></author><author><style face="normal" font="default" size="100%">NOZAKA, MASASHI</style></author><author><style face="normal" font="default" size="100%">KAWAMURA, YOSUKE</style></author><author><style face="normal" font="default" size="100%">FUJITA, TAKESHI</style></author><author><style face="normal" font="default" size="100%">MURAKAMI, REIICHI</style></author><author><style face="normal" font="default" size="100%">IMAIZUMI, TADAATSU</style></author><author><style face="normal" font="default" size="100%">NAKAMURA, NORIO</style></author><author><style face="normal" font="default" size="100%">TOMITA, HIROFUMI</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">PolyIC Induces Retinoic Acid-inducible Gene-I and Melanoma Differentiation-associated Gene 5 and Modulates Inflammation in Podocytes</style></title><secondary-title><style face="normal" font="default" size="100%">In Vivo</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2021-01-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">147-153</style></pages><doi><style  face="normal" font="default" size="100%">10.21873/invivo.12242</style></doi><volume><style face="normal" font="default" size="100%">35</style></volume><issue><style face="normal" font="default" size="100%">1</style></issue><abstract><style  face="normal" font="default" size="100%">Background/Aim: Viral infection often exacerbates proteinuria, which has been suggested to be due to antiviral responses of podocytes. We examined the effect of polyinosinic-polycytidylic acid (polyIC) on the expression of retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5) in differentiated human podocytes in culture. Materials and Methods: The podocytes were treated with 2 ng/ml to 500 μg/ml of polyIC for 3 to 36 h, and also transfected with siRNA against RIG-I and MDA5. F-actin staining was performed to assess actin reorganization. Results: PolyIC induced the expression of RIG-I and MDA5 in dose- and time-dependent manner, accompanied with interferon-β (IFN-β) and interleukin-6 (IL-6) up-regulation and actin reorganization. Temporal knockdown of RIG-I by siRNA decreased IFN-β expression, while MDA5 siRNA inhibited IFN-β and IL-6 expression. Actin reorganization was attenuated by RIG-I and MDA5 knockdown. Conclusion: RIG-I and MDA5 may play a role in the antiviral responses of podocytes.</style></abstract></record></records></xml>