TY - JOUR T1 - Tumor Growth Suppression With Novel Intra-arterial Chemotherapy Using Epirubicin-entrapped Water-in-oil-in-water Emulsion <em>In Vivo</em> JF - In Vivo JO - In Vivo SP - 239 LP - 248 DO - 10.21873/invivo.12252 VL - 35 IS - 1 AU - HIRONOBU YANAGIE AU - TAKASHI FUJINO AU - MASASHI YANAGAWA AU - TOSHIMITSU TERAO AU - TAKASHI IMAGAWA AU - MITSUTERU FUJIHARA AU - YASUYUKI MORISHITA AU - RYOUJI MIZUMACHI AU - YUUJI MURATA AU - NOVRIANA DEWI AU - YUUYA ONO AU - ICHIRO IKUSHIMA AU - KOJI SEGUCHI AU - MASASHI NAGATA AU - YASUMASA NONAKA AU - YOSHITAKA FURUYA AU - TOMOYUKI HISA AU - TAKESHI NAGASAKI AU - KAZUHIKO ARIMORI AU - TADAO NAKASHIMA AU - TAKUMICHI SUGIHARA AU - KAZUHIRO KAKIMI AU - MINORU ONO AU - JUN NAKAJIMA AU - MASAZUMI ERIGUCHI AU - SHUSHI HIGASHI AU - HIROYUKI TAKAHASHI Y1 - 2021/01/01 UR - http://iv.iiarjournals.org/content/35/1/239.abstract N2 - Background/Aim: A mixture of anticancer agents and iodized poppy seed oil (IPSO) has been widely used for intra-arterial chemotherapy of hepatocellular carcinoma. However, the anticancer agents can easily separate from IPSO, so the therapeutic potential is limited. We developed epirubicin-entrapped water-in-oil-in-water emulsion (WOW-Epi) using a double-membrane emulsification technique. Materials and Methods: We delivered WOW-Epi through a hepatic arterial injection to VX2 hepatic tumor rabbit model (1.2 mg/kg). Results: VX2 tumor growth was selectively suppressed in the WOW-Epi-treated group compared with the control treated groups. The accumulation of WOW in nearby cancer cells was confirmed via electron-microscopy. Endocytosis seemed to be the mechanism underlying the uptake of WOW. Conclusion: WOW-Epi led to tumour growth suppression in vivo. WOW does not cause toxicity to arterial vessels. WOW-Epi will be hopefully used for repeated intra-arterial chemotherapy to HCC patients in the near future. ER -