TY - JOUR T1 - Relationship Between Insulin-Receptor Substrate 1 and Langerhans’ Islet in a Rat Model of Type 2 Diabetes Mellitus JF - In Vivo JO - In Vivo SP - 291 LP - 297 DO - 10.21873/invivo.12258 VL - 35 IS - 1 AU - WAHYUNI LUKITA ATMODJO AU - YOUNG OTHIWI LARASATI AU - JUANDY JO AU - RISKA NUFIKA AU - STEFFI NAOMI AU - IMELDA WINOTO Y1 - 2021/01/01 UR - http://iv.iiarjournals.org/content/35/1/291.abstract N2 - Background/Aim: In vivo studies on pathogenesis of type 2 diabetes mellitus (T2DM) have been reported, however, the relationship between insulin-receptor substrate 1 (IRS1) and the area of Langerhans’ islets was unknown. Therefore, a correlation between both parameters was assessed. Materials and Methods: Diabetic groups were fed with a high-fat diet (HFD) and injected with three different doses of streptozotocin, namely 25, 35 and 45 mg/kg, and compared to a control group after 9 weeks. Results: Administration of HFD/streptozotocin increased the level of fasting blood glucose but reduced the level of IRS1 and the area of Langerhans’ islets in diabetic groups. The coefficient of correlation between IRS1 and area of Langerhans’ islets was 0.259 (p=0.232). In addition, the coefficient of correlation for fasting blood glucose with the area of Langerhans’ islets and IRS1 was −0.520 (p=0.011) and −0.603 (p=0.002), respectively. Conclusion: The reduction of IRS1 was weakly correlated with the destruction of Langerhans’ islets, suggesting there is an intermediate step between both parameters. ER -