PT  - JOURNAL ARTICLE
AU  - TAKUYA MURATA
AU  - CHIHIRO HOZUMI
AU  - YUKIHIKO HIROSHIMA
AU  - KOICHIRO SHIMOYA
AU  - ATSUSHI HONGO
AU  - SACHIKO INUBUSHI
AU  - HIROKAZU TANINO
AU  - ROBERT M. HOFFMAN
TI  - Co-implantation of Tumor and Extensive Surrounding Tissue Improved the Establishment Rate of Surgical Specimens of Human-Patient Cancer in Nude Mice: Toward the Goal of Universal Individualized Cancer Therapy
AID  - 10.21873/invivo.12160
DP  - 2020 Nov 01
TA  - In Vivo
PG  - 3241--3245
VI  - 34
IP  - 6
4099  - http://iv.iiarjournals.org/content/34/6/3241.short
4100  - http://iv.iiarjournals.org/content/34/6/3241.full
SO  - In Vivo2020 Nov 01; 34
AB  - Background/Aim: The discovery of the nude mouse model enabled the experimental growth of human-patient tumors. However, the low establishment rate of tumors in nude and other immunodeficient strains of mice has limited wide-spread clinical use. Materials and Methods: In order to increase the establishment rate of surgical specimens of patient tumors, we transplanted tumors to nude mice subcutaneously along with large amounts of surrounding tissue of the tumor. Results: The new transplantation method increased the establishment rate in nude mice to 66% compared to the old method of implanting the surgical tumor specimen with surrounding tissue removed (14%). High stage and presence of metastasis in the patient donor are positively correlated to tumor engraftment in nude mice. Conclusion: The new method can potentially allow most cancer patients who undergo surgery or biopsy to have their own mouse model for drug-sensitivity testing.