%0 Journal Article %A TAKUYA MURATA %A CHIHIRO HOZUMI %A YUKIHIKO HIROSHIMA %A KOICHIRO SHIMOYA %A ATSUSHI HONGO %A SACHIKO INUBUSHI %A HIROKAZU TANINO %A ROBERT M. HOFFMAN %T Co-implantation of Tumor and Extensive Surrounding Tissue Improved the Establishment Rate of Surgical Specimens of Human-Patient Cancer in Nude Mice: Toward the Goal of Universal Individualized Cancer Therapy %D 2020 %R 10.21873/invivo.12160 %J In Vivo %P 3241-3245 %V 34 %N 6 %X Background/Aim: The discovery of the nude mouse model enabled the experimental growth of human-patient tumors. However, the low establishment rate of tumors in nude and other immunodeficient strains of mice has limited wide-spread clinical use. Materials and Methods: In order to increase the establishment rate of surgical specimens of patient tumors, we transplanted tumors to nude mice subcutaneously along with large amounts of surrounding tissue of the tumor. Results: The new transplantation method increased the establishment rate in nude mice to 66% compared to the old method of implanting the surgical tumor specimen with surrounding tissue removed (14%). High stage and presence of metastasis in the patient donor are positively correlated to tumor engraftment in nude mice. Conclusion: The new method can potentially allow most cancer patients who undergo surgery or biopsy to have their own mouse model for drug-sensitivity testing. %U https://iv.iiarjournals.org/content/invivo/34/6/3241.full.pdf