@article {MURATA3241, author = {TAKUYA MURATA and CHIHIRO HOZUMI and YUKIHIKO HIROSHIMA and KOICHIRO SHIMOYA and ATSUSHI HONGO and SACHIKO INUBUSHI and HIROKAZU TANINO and ROBERT M. HOFFMAN}, title = {Co-implantation of Tumor and Extensive Surrounding Tissue Improved the Establishment Rate of Surgical Specimens of Human-Patient Cancer in Nude Mice: Toward the Goal of Universal Individualized Cancer Therapy}, volume = {34}, number = {6}, pages = {3241--3245}, year = {2020}, doi = {10.21873/invivo.12160}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: The discovery of the nude mouse model enabled the experimental growth of human-patient tumors. However, the low establishment rate of tumors in nude and other immunodeficient strains of mice has limited wide-spread clinical use. Materials and Methods: In order to increase the establishment rate of surgical specimens of patient tumors, we transplanted tumors to nude mice subcutaneously along with large amounts of surrounding tissue of the tumor. Results: The new transplantation method increased the establishment rate in nude mice to 66\% compared to the old method of implanting the surgical tumor specimen with surrounding tissue removed (14\%). High stage and presence of metastasis in the patient donor are positively correlated to tumor engraftment in nude mice. Conclusion: The new method can potentially allow most cancer patients who undergo surgery or biopsy to have their own mouse model for drug-sensitivity testing.}, issn = {0258-851X}, URL = {https://iv.iiarjournals.org/content/34/6/3241}, eprint = {https://iv.iiarjournals.org/content/34/6/3241.full.pdf}, journal = {In Vivo} }