TY - JOUR T1 - The MSC-MCF-7 Duet Playing Tumor Vasculogenesis and Angiogenesis onto the Chick Embryo Chorioallantoic Membrane JF - In Vivo JO - In Vivo SP - 3315 LP - 3325 DO - 10.21873/invivo.12170 VL - 34 IS - 6 AU - ŞERBAN COMŞA AU - AMALIA-RALUCA CEAUȘU AU - ROXANA POPESCU AU - SIMONA SÂRB AU - ANCA-MARIA CÎMPEAN AU - MARIUS RAICA Y1 - 2020/11/01 UR - http://iv.iiarjournals.org/content/34/6/3315.abstract N2 - Background/Aim: Human mesenchymal stem cells (hMSC) represent a versatile cell population, able to modulate the tumor microenvironment. Our aim was to recreate an open scene for the in vivo interaction between hMSC and the MCF-7 breast cancer cells (MCF-7), in order to enlighten the intimate involvement of hMSC in tumor vasculogenesis and angiogenesis. Materials and Methods: hMSC and MCF-7 were seeded onto the chick embryo chorioallantoic membrane (CAM) and incubated for 7 days. Consecutively, the morphology and the immunohistochemical profile of CAM were assessed. Results: Following this complex interaction, MCF-7 acquired a more aggressive phenotype, hMSC switched to a vascular precursor phenotype, while CAM underwent a major reset to an earlier stage, with hotspots of angiogenesis, vasculogenesis and hematopoiesis. Conclusion: The hallmark of this study was the establishment of a veritable in vivo experimental model of MSC involvement in tumor vasculogenesis and angiogenesis, allowing further analysis in the field. ER -