TY - JOUR T1 - A Non-invasive Imageable GFP-expressing Mouse Model of Orthotopic Human Bladder Cancer JF - In Vivo JO - In Vivo SP - 3225 LP - 3231 DO - 10.21873/invivo.12158 VL - 34 IS - 6 AU - YU SUN AU - HIROTO NISHINO AU - MING ZHAO AU - KENTARO MIYAKE AU - NORIHIKO SUGISAWA AU - JUN YAMAMOTO AU - YOSHIHIKO TASHIRO AU - SACHIKO INUBUSHI AU - KAZUYUKI HAMADA AU - GUANGWEI ZHU AU - HYEIN LIM AU - ROBERT M. HOFFMAN Y1 - 2020/11/01 UR - http://iv.iiarjournals.org/content/34/6/3225.abstract N2 - Background/Aim: A more realistic mouse model of bladder cancer is necessary to develop effective drugs for the disease. Tumor models enhanced by bright fluorescent-reporter genes to follow the disease in real-time would enhance the ability to accurately predict the efficacy of various therapeutics on this particularly-malignant human cancer. Materials and Methods: A highly-fluorescent green fluorescent protein (GFP)-expressing bladder cancer model was orthotopically established in nude mice using the UM-UC-3 human bladder-cancer cell line (UM-UC-3-GFP). Fragments from a subcutaneous tumor of UM-UC-3-GFP were surgically implanted into the nude mouse bladder. Non-invasive and intra-vital fluorescence imaging was obtained with a simple imaging box. Results: The GFP-expressing orthotopic bladder tumor was imaged in real-time non-invasively as well as intra-vitally, with the two methods correlating at r=0.99. Conclusion: This is the first non-invasive-fluorescence-imaging orthotopic model of bladder cancer and can be used for rapidly screening novel effective agents for this recalcitrant disease. ER -