TY - JOUR T1 - Cryopreservation of Biologically Functional Submandibular Gland Rudiments from Fetal Mice JF - In Vivo JO - In Vivo SP - 3271 LP - 3277 DO - 10.21873/invivo.12164 VL - 34 IS - 6 AU - KEISUKE ADACHI AU - YUTA OHNO AU - KEITARO SATOH AU - AKIKO SHITARA AU - YASUNORI MURAMATHU AU - MASANORI KASHIMATA Y1 - 2020/11/01 UR - http://iv.iiarjournals.org/content/34/6/3271.abstract N2 - Background/Aim: Cryopreservation of cell lines has been widely used in the laboratory; however, cryopreservation of organs is still considered to be difficult. The submandibular gland (SMG) of fetal mice is one of the best-characterized organs. We investigated the conditions for cryopreserving SMG rudiments. Materials and Methods: Embryonic day 13 SMG rudiments were cryopreserved with or without a cryoprotectant. They were thawed and incubated in DMEM/F12 medium. Moreover, the influence of EGF stimulation on the signaling cascade after frozen-thawing the rudiments was analyzed by Western blotting. Results: When SMG rudiments were cryopreserved without a cryoprotectant, all cells in the rudiments died. However, the SMG rudiments that had been preserved in a cryoprotectant showed branching morphogenesis. Additionally, the responsiveness of signaling cascades to EGF did not differ between frozen with a cryoprotectant and non-frozen rudiments. Conclusion: Cryopreservation might be a useful technology for preserving tissues from small organs, such as fetal SMG rudiments. ER -