RT Journal Article
SR Electronic
T1 Sutureless Surgical Orthotopic Implantation Technique of Primary and Metastatic Cancer in the Liver of Mouse Models
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 3153
OP 3157
DO 10.21873/invivo.12149
VO 34
IS 6
A1 HIROTO NISHINO
A1 HANNAH M. HOLLANDSWORTH
A1 NORIHIKO SUGISAWA
A1 JUN YAMAMOTO
A1 YOSHIHIKO TASHIRO
A1 SACHIKO INUBUSHI
A1 KAZUYUKI HAMADA
A1 YU SUN
A1 HYEIN LIM
A1 SIAMAK AMIRFAKHRI
A1 FILEMONI FILEMONI
A1 ROBERT M. HOFFMAN
A1 MICHAEL BOUVET
YR 2020
UL http://iv.iiarjournals.org/content/34/6/3153.abstract
AB Background/Aim: Surgical orthotopic implantation (SOI) is used to establish patient-derived orthotopic xenograft (PDOX) and other orthotopic mouse models. Orthotopic liver models can be challenging, as the liver parenchyma is prone to bleeding. The present report describes a sutureless method to implant tumors in the liver that reduces bleeding and procedural time. Materials and Methods: Human HCC cell-line (Huh-7-GFP) and CM2, a patient-derived colon-cancer liver metastasis, were used for sutureless SOI of tumor fragments in the liver of nude mice. A small cavity was formed on the liver surface. A solitary tumor fragment was implanted in the cavity without suturing to create hemostasis. Results: Six weeks after sutureless SOI, the tumor volume of Huh-7-GFP (n=5) was 584.41±147.64 mm3 and the tumor volume of CM2 (n=5) was 1336.54±1038.20 mm3. The engraftment rate was 100%. Conclusion: This novel method for establishing orthotopic liver-implantation mouse models is suitable for studies of liver cancer and liver metastases due to its simple procedure and potential high engraftment rate.