PT - JOURNAL ARTICLE AU - HIROTO NISHINO AU - HANNAH M. HOLLANDSWORTH AU - NORIHIKO SUGISAWA AU - JUN YAMAMOTO AU - YOSHIHIKO TASHIRO AU - SACHIKO INUBUSHI AU - KAZUYUKI HAMADA AU - YU SUN AU - HYEIN LIM AU - SIAMAK AMIRFAKHRI AU - FILEMONI FILEMONI AU - ROBERT M. HOFFMAN AU - MICHAEL BOUVET TI - Sutureless Surgical Orthotopic Implantation Technique of Primary and Metastatic Cancer in the Liver of Mouse Models AID - 10.21873/invivo.12149 DP - 2020 Nov 01 TA - In Vivo PG - 3153--3157 VI - 34 IP - 6 4099 - http://iv.iiarjournals.org/content/34/6/3153.short 4100 - http://iv.iiarjournals.org/content/34/6/3153.full SO - In Vivo2020 Nov 01; 34 AB - Background/Aim: Surgical orthotopic implantation (SOI) is used to establish patient-derived orthotopic xenograft (PDOX) and other orthotopic mouse models. Orthotopic liver models can be challenging, as the liver parenchyma is prone to bleeding. The present report describes a sutureless method to implant tumors in the liver that reduces bleeding and procedural time. Materials and Methods: Human HCC cell-line (Huh-7-GFP) and CM2, a patient-derived colon-cancer liver metastasis, were used for sutureless SOI of tumor fragments in the liver of nude mice. A small cavity was formed on the liver surface. A solitary tumor fragment was implanted in the cavity without suturing to create hemostasis. Results: Six weeks after sutureless SOI, the tumor volume of Huh-7-GFP (n=5) was 584.41±147.64 mm3 and the tumor volume of CM2 (n=5) was 1336.54±1038.20 mm3. The engraftment rate was 100%. Conclusion: This novel method for establishing orthotopic liver-implantation mouse models is suitable for studies of liver cancer and liver metastases due to its simple procedure and potential high engraftment rate.