%0 Journal Article %A HIROTO NISHINO %A HANNAH M. HOLLANDSWORTH %A NORIHIKO SUGISAWA %A JUN YAMAMOTO %A YOSHIHIKO TASHIRO %A SACHIKO INUBUSHI %A KAZUYUKI HAMADA %A YU SUN %A HYEIN LIM %A SIAMAK AMIRFAKHRI %A FILEMONI FILEMONI %A ROBERT M. HOFFMAN %A MICHAEL BOUVET %T Sutureless Surgical Orthotopic Implantation Technique of Primary and Metastatic Cancer in the Liver of Mouse Models %D 2020 %R 10.21873/invivo.12149 %J In Vivo %P 3153-3157 %V 34 %N 6 %X Background/Aim: Surgical orthotopic implantation (SOI) is used to establish patient-derived orthotopic xenograft (PDOX) and other orthotopic mouse models. Orthotopic liver models can be challenging, as the liver parenchyma is prone to bleeding. The present report describes a sutureless method to implant tumors in the liver that reduces bleeding and procedural time. Materials and Methods: Human HCC cell-line (Huh-7-GFP) and CM2, a patient-derived colon-cancer liver metastasis, were used for sutureless SOI of tumor fragments in the liver of nude mice. A small cavity was formed on the liver surface. A solitary tumor fragment was implanted in the cavity without suturing to create hemostasis. Results: Six weeks after sutureless SOI, the tumor volume of Huh-7-GFP (n=5) was 584.41±147.64 mm3 and the tumor volume of CM2 (n=5) was 1336.54±1038.20 mm3. The engraftment rate was 100%. Conclusion: This novel method for establishing orthotopic liver-implantation mouse models is suitable for studies of liver cancer and liver metastases due to its simple procedure and potential high engraftment rate. %U https://iv.iiarjournals.org/content/invivo/34/6/3153.full.pdf