PT  - JOURNAL ARTICLE
AU  - KOSUKE YAMADA
AU  - TAKESHI AOKI
AU  - YUTA ENAMI
AU  - YOSHIHIKO TASHIRO
AU  - ZIN ZEHAOU
AU  - TOMOTAKE KOIZUMI
AU  - TOMOKAZU KUSANO
AU  - KAZUHIRO MATSUDA
AU  - YUSUKE WADA
AU  - HIDEKI SHIBATA
AU  - KODAI TOMIOKA
AU  - KRIS SIRIRATSIVAWONG
AU  - ROBERT M. HOFFMAN
AU  - MASAHIKO MURAKAMI
TI  - An Improved Encapsulation Method for Cryopreserving Hepatocytes for Functional Transplantation Using a Thermo-reversible Gelation Polymer
AID  - 10.21873/invivo.12043
DP  - 2020 Sep 01
TA  - In Vivo
PG  - 2309--2316
VI  - 34
IP  - 5
4099  - http://iv.iiarjournals.org/content/34/5/2309.short
4100  - http://iv.iiarjournals.org/content/34/5/2309.full
SO  - In Vivo2020 Sep 01; 34
AB  - Background/Aim: Thermo-reversible gelation polymer (TGP) can be converted into a gel state upon warming and liquid upon cooling. The present study aimed to demonstrate a new method for cryopreservation and encapsulation of rat hepatocytes using a TGP and their successful transplantation. Materials and Methods: The isolated rat hepatocytes were microencapsulated using TGP, and stored in liquid nitrogen. After cryopreservation, hepatocytes were cultured. Moreover, hepatocytes were transplanted into the spleen without a TGP capsule. Results: The viability of hepatocytes that were cryopreserved in TGP was 71.2±2.3%. The hepatocytes demonstrated adequate survival, maintained their hepatic function in culture, and expressed albumin after transplantation to the rat spleen. Conclusion: We demonstrated a cryopreservation method of rat hepatocyte encapsulation using a TGP gel in the hydrogel state which subsequently allowed successful transplantation of unencapsulated hepatocytes in a sol state TGP gel at low temperature.