TY - JOUR T1 - Expression of Ascorbate Peroxidase Derived from <em>Cyanidioschyzon merolae</em> in Mammalian Cells JF - In Vivo JO - In Vivo SP - 2437 LP - 2441 DO - 10.21873/invivo.12058 VL - 34 IS - 5 AU - SUZURO HITOMI AU - SHOICHIRO KOKABU AU - KEN-ICHIRO MATSUMOTO AU - YOSHIMI SHOJI AU - IZUMI UJIHARA AU - KENTARO ONO Y1 - 2020/09/01 UR - http://iv.iiarjournals.org/content/34/5/2437.abstract N2 - Background/Aim: Ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, a primitive red alga living in high temperature and acidic environments, has greater anti-oxidative capacity than similar peroxidases occurring in other plants. In the present study, we examined whether expression of Cyanidioschyzon merolae-derived APX (cAPX) in mammalian cells increases cellular anti-oxidative capacity. Materials and Methods: The cAPX gene was introduced into the mouse fibroblast-like cell line C3H10T1/2. Production of reactive oxygen species (ROS) and/or cell viability was assessed after heat, H2O2 and acid stimulation. Results: Heat and H2O2 stimulation resulted in ROS production. cAPX-expressing cells were more tolerant to oxidative stress induced by heat, H2O2 and acid stimulations than control cells lacking cAPX. Conclusion: Introduction of cAPX increases the anti-oxidative capacity in mammalian cells. ER -