RT Journal Article SR Electronic T1 Expression of Ascorbate Peroxidase Derived from Cyanidioschyzon merolae in Mammalian Cells JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 2437 OP 2441 DO 10.21873/invivo.12058 VO 34 IS 5 A1 HITOMI, SUZURO A1 KOKABU, SHOICHIRO A1 MATSUMOTO, KEN-ICHIRO A1 SHOJI, YOSHIMI A1 UJIHARA, IZUMI A1 ONO, KENTARO YR 2020 UL http://iv.iiarjournals.org/content/34/5/2437.abstract AB Background/Aim: Ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, a primitive red alga living in high temperature and acidic environments, has greater anti-oxidative capacity than similar peroxidases occurring in other plants. In the present study, we examined whether expression of Cyanidioschyzon merolae-derived APX (cAPX) in mammalian cells increases cellular anti-oxidative capacity. Materials and Methods: The cAPX gene was introduced into the mouse fibroblast-like cell line C3H10T1/2. Production of reactive oxygen species (ROS) and/or cell viability was assessed after heat, H2O2 and acid stimulation. Results: Heat and H2O2 stimulation resulted in ROS production. cAPX-expressing cells were more tolerant to oxidative stress induced by heat, H2O2 and acid stimulations than control cells lacking cAPX. Conclusion: Introduction of cAPX increases the anti-oxidative capacity in mammalian cells.