PT  - JOURNAL ARTICLE
AU  - SUZURO HITOMI
AU  - SHOICHIRO KOKABU
AU  - KEN-ICHIRO MATSUMOTO
AU  - YOSHIMI SHOJI
AU  - IZUMI UJIHARA
AU  - KENTARO ONO
TI  - Expression of Ascorbate Peroxidase Derived from <em>Cyanidioschyzon merolae</em> in Mammalian Cells
AID  - 10.21873/invivo.12058
DP  - 2020 Sep 01
TA  - In Vivo
PG  - 2437--2441
VI  - 34
IP  - 5
4099  - http://iv.iiarjournals.org/content/34/5/2437.short
4100  - http://iv.iiarjournals.org/content/34/5/2437.full
SO  - In Vivo2020 Sep 01; 34
AB  - Background/Aim: Ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, a primitive red alga living in high temperature and acidic environments, has greater anti-oxidative capacity than similar peroxidases occurring in other plants. In the present study, we examined whether expression of Cyanidioschyzon merolae-derived APX (cAPX) in mammalian cells increases cellular anti-oxidative capacity. Materials and Methods: The cAPX gene was introduced into the mouse fibroblast-like cell line C3H10T1/2. Production of reactive oxygen species (ROS) and/or cell viability was assessed after heat, H2O2 and acid stimulation. Results: Heat and H2O2 stimulation resulted in ROS production. cAPX-expressing cells were more tolerant to oxidative stress induced by heat, H2O2 and acid stimulations than control cells lacking cAPX. Conclusion: Introduction of cAPX increases the anti-oxidative capacity in mammalian cells.