RT Journal Article SR Electronic T1 Effect of 7,12-Dimethylbenz(α)anthracene on the Expression of miR-330, miR-29a, miR-9-1, miR-9-3 and the mTORC1 Gene in CBA/Ca Mice JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 2337 OP 2343 DO 10.21873/invivo.12046 VO 34 IS 5 A1 ANDRAS TOMESZ A1 LASZLO SZABO A1 RICHARD MOLNAR A1 ARPAD DEUTSCH A1 RICHARD DARAGO A1 DOMOKOS MATHE A1 FERENC BUDAN A1 NOWRASTEH GHODRATOLLAH A1 TIMEA VARJAS A1 BALAZS NEMETH A1 ISTVAN KISS YR 2020 UL http://iv.iiarjournals.org/content/34/5/2337.abstract AB Background/Aim: Development of malignant tumors is preceded by molecular biological events. Our aim was to establish an assay panel by using miRNAs and other genes for the rapid screening of potential carcinogens or chemopreventive agents. Materials and Methods: Six male and 6 female CBA/Ca mice received 20 mg/bwkg 7,12-dimethylbenz(α)anthracene (DMBA) intraperitoneally, and 24 h later RNA was isolated from parenchymal organs. Expression of miR-330, miR-29a, miR-9-1, miR-9-3 and mTORC1 was analysed by real time polymerase chain reaction and compared to non-treated controls. Results: DMBA caused significant alterations in the expression of the studied genes. The most profound changes were the strongly elevated miR-9-3 and mTORC1 expressions in female mice in all organs studied. Conclusion: miR-9-3 and mTORC1 expression in female mice were found to be the most suitable biomarkers for rapid identification of possible carcinogenic effects.