PT  - JOURNAL ARTICLE
AU  - ANDRAS TOMESZ
AU  - LASZLO SZABO
AU  - RICHARD MOLNAR
AU  - ARPAD DEUTSCH
AU  - RICHARD DARAGO
AU  - DOMOKOS MATHE
AU  - FERENC BUDAN
AU  - NOWRASTEH GHODRATOLLAH
AU  - TIMEA VARJAS
AU  - BALAZS NEMETH
AU  - ISTVAN KISS
TI  - Effect of 7,12-Dimethylbenz(α)anthracene on the Expression of miR-330, miR-29a, miR-9-1, miR-9-3 and the mTORC1 Gene in CBA/Ca Mice
AID  - 10.21873/invivo.12046
DP  - 2020 Sep 01
TA  - In Vivo
PG  - 2337--2343
VI  - 34
IP  - 5
4099  - http://iv.iiarjournals.org/content/34/5/2337.short
4100  - http://iv.iiarjournals.org/content/34/5/2337.full
SO  - In Vivo2020 Sep 01; 34
AB  - Background/Aim: Development of malignant tumors is preceded by molecular biological events. Our aim was to establish an assay panel by using miRNAs and other genes for the rapid screening of potential carcinogens or chemopreventive agents. Materials and Methods: Six male and 6 female CBA/Ca mice received 20 mg/bwkg 7,12-dimethylbenz(α)anthracene (DMBA) intraperitoneally, and 24 h later RNA was isolated from parenchymal organs. Expression of miR-330, miR-29a, miR-9-1, miR-9-3 and mTORC1 was analysed by real time polymerase chain reaction and compared to non-treated controls. Results: DMBA caused significant alterations in the expression of the studied genes. The most profound changes were the strongly elevated miR-9-3 and mTORC1 expressions in female mice in all organs studied. Conclusion: miR-9-3 and mTORC1 expression in female mice were found to be the most suitable biomarkers for rapid identification of possible carcinogenic effects.