RT Journal Article
SR Electronic
T1 Indocyanine Green Labels an Orthotopic Nude-Mouse Model of Very-Early Colon-Cancer Liver Metastases
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 2277
OP 2280
DO 10.21873/invivo.12038
VO 34
IS 5
A1 TASHIRO, YOSHIHIKO
A1 HOLLANDSWORTH, HANNAH M.
A1 NISHINO, HIROTO
A1 YAMAMOTO, JUN
A1 AMIRFAKHRI, SIAMAK
A1 FILEMONI, FILEMONI
A1 SUGISAWA, NORIHIKO
A1 AOKI, TAKESHI
A1 MURAKAMI, MASAHIKO
A1 HOFFMAN, ROBERT M.
A1 BOUVET, MICHAEL
YR 2020
UL http://iv.iiarjournals.org/content/34/5/2277.abstract
AB Background/Aim: Near-infrared (NIR) fluorescence imaging using indocyanine green (ICG) is used to visualize colon-cancer liver metastases (CCLM) during surgery. The present study aimed to use ICG to visualize small CCLM in an orthotopic mouse model. Materials and Methods: A 3-mm fragment of HT29 human colon cancer was implanted to the liver of 5 athymic nude mice (nu/nu). The Pearl Trilogy Small Animal Fluorescence Imaging system was used 24 h after intravenous (IV) injection of 0.025 mg (0.25 mg/ml) ICG. Results: In four of the five mice, tumor fluorescence was detected. Small tumors (approximately 3 mm) were distinctly visualized with a minimal fluorescence liver signal, with a mean tumor to liver signal ratio of 1.81 (SD±0.167). Conclusion: Small CCLM can be clearly identified by ICG fluorescence in an orthotopic nude-mouse model. This model is translatable to the clinic for the detection of occult early CCLM.