RT Journal Article
SR Electronic
T1 Deep Sequencing Identified Dysregulated Circulating MicroRNAs in Late Onset Preeclampsia
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 2317
OP 2324
DO 10.21873/invivo.12044
VO 34
IS 5
A1 DANAI MAVRELI
A1 ALEXANDRA LYKOUDI
A1 GEORGE LAMBROU
A1 GEORGE PAPAIOANNOU
A1 NIKOLAS VRACHNIS
A1 SOPHIA KALANTARIDOU
A1 NIKOLAS PAPANTONIOU
A1 AGGELIKI KOLIALEXI
YR 2020
UL http://iv.iiarjournals.org/content/34/5/2317.abstract
AB Background/Aim: To characterize global microRNA (miRNA) expression profile in the first trimester maternal plasma of women who subsequently develop late-onset preeclampsia (LOPE) compared to uncomplicated pregnancies. Materials and Methods: Five first trimester plasma samples from women who developed LOPE and 5 controls were analyzed using next generation sequencing technology (NGS) followed by target prediction, Gene Ontology analysis and pathway identification. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed for confirmation in an independent cohort of 12 LOPE cases and 12 controls. Results: miR-23b-5p and miR-99b-5p were down-regulated by >1.5 fold in LOPE complicated pregnancies (p value <0.05) compared to controls. Target prediction showed that the major targets of these miRNAs are associated with glycometabolism and immune response. Conclusion: miR-23b-5p and miR-99b-5p are possibly implicated in the pathogenic mechanisms leading to the induction of LOPE and may serve as candidate non-invasive biomarkers for early prediction and prevention.