@article {ENOMOTO2297, author = {HIRAYUKI ENOMOTO and HIDEJI NAKAMURA and HIROKI NISHIKAWA and TAKASHI NISHIMURA and YOSHINORI IWATA and SHUHEI NISHIGUCHI and HIROKO IIJIMA}, title = {Hepatocellular Carcinoma-associated microRNAs Induced by Hepatoma-derived Growth Factor Stimulation}, volume = {34}, number = {5}, pages = {2297--2301}, year = {2020}, doi = {10.21873/invivo.12041}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: Hepatoma-derived growth factor (HDGF) is involved in the progression of hepatocellular carcinoma (HCC). The present study assessed the epigenomic changes in hepatoma-derived cells through HDGF stimulation. Materials and Methods: We used two hepatoma-derived cell lines (HepG2 and SK-Hep1) and searched for microRNAs whose expression commonly changed in response to HDGF administration. We further explored a genetic database to investigate the association of the candidate microRNAs with the survival of HCC patients. Results: Despite both HepG2 and SK-Hep1 cells being categorized as hepatoma-derived cells, the microRNA profile differed between these two lines. However, HepG2 and SK-Hep1 cells shared 30 up-regulated and 2 down-regulated microRNAs. Of these, miR-6072 and miR-3137 were significantly associated with a poor prognosis in HCC patients. Conclusion: We identified two candidate microRNAs whose expression increased in response to HDGF stimulation. Both these molecules were associated with a poor prognosis of HCC patients.}, issn = {0258-851X}, URL = {https://iv.iiarjournals.org/content/34/5/2297}, eprint = {https://iv.iiarjournals.org/content/34/5/2297.full.pdf}, journal = {In Vivo} }