RT Journal Article
SR Electronic
T1 Humanized Fluorescent Tumor-associated Glycoprotein-72 Antibody Selectively Labels Colon-cancer Liver Metastases in Orthotopic Mouse Models
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 2303
OP 2307
DO 10.21873/invivo.12042
VO 34
IS 5
A1 HANNAH M. HOLLANDSWORTH
A1 HIROTO NISHINO
A1 MICHAEL TURNER
A1 SIAMAK AMIRFAKHRI
A1 FILEMONI FILEMONI
A1 ROBERT M. HOFFMAN
A1 PAUL J. YAZAKI
A1 MICHAEL BOUVET
YR 2020
UL http://iv.iiarjournals.org/content/34/5/2303.abstract
AB Background/Aim: Fluorescence imaging has been shown to improve intra-operative detection of liver metastasis. The present study aimed to determine whether humanized anti-TAG-72 antibody (huCC49) conjugated to a near-infrared dye provides selective labeling of colorectal-cancer liver metastasis in orthotopic mouse models. Materials and Methods: Humanized anti-TAG-72 (huCC49) was conjugated to IRDye800CW (huCC49-IR800). Orthotopic liver-metastasis nude-mouse models (n=5) were established with the human colon-cancer LS174T cell-line. Three weeks later, mice were administered huCC49-IR800 and intra-vital imaging was performed 48 h later. The mean tumor-to-liver ratio (TLR) was calculated. Results: Intra-vital imaging demonstrated clear tumor margins with minimal liver fluorescence 48 h after administration of 50 μg huCC49-IR800 with mean TLR=7.53 (SD±2.76). Conclusion: Anti-TAG-72 monoclonal antibody conjugated to IRDye800 provides distinct and bright labeling of colorectal tumors in orthotopic nude-mouse models of liver metastasis. TAG-72 may be a useful target for intra-operative imaging of colorectal cancer liver metastasis in the clinic.