PT - JOURNAL ARTICLE AU - SEI UEDA AU - KENGO HASHIMOTO AU - SATORU MIYABE AU - SHOGO HASEGAWA AU - MITSUO GOTO AU - DAI SHIMIZU AU - ICHIRO OH-IWA AU - KAZUO SHIMOZATO AU - TORU NAGAO AU - SHUJI NOMOTO TI - Salivary <em>NUS1</em> and <em>RCN1</em> Levels as Biomarkers for Oral Squamous Cell Carcinoma Diagnosis AID - 10.21873/invivo.12048 DP - 2020 Sep 01 TA - In Vivo PG - 2353--2361 VI - 34 IP - 5 4099 - http://iv.iiarjournals.org/content/34/5/2353.short 4100 - http://iv.iiarjournals.org/content/34/5/2353.full SO - In Vivo2020 Sep 01; 34 AB - Background/Aim: Oral cancer may become advanced because of delay in diagnosis. In order to promote oral cancer screening, simple and highly reliable screening methods that can be implemented at general dental clinics are required. Herein we investigated differential salivary gene expression between oral squamous cell carcinoma (OSCC) patients and healthy volunteers (HV) to identify new biomarkers for OSCC detection. Materials and Methods: Candidate genes were selected by microarrays, nuclear undecaprenyl pyrophosphate synthase 1 (NUS1) and reticulocalbin 1 (RCN1) were selected for further investigation. We used real-time quantitative reverse transcription PCR (qRT-PCR) to determine NUS1 and RCN1 expression levels in saliva and tissues. Results: qRT-PCR analysis of clinical samples revealed that OSCC patients had significantly higher expression of salivary NUS1 and RCN1 than HV. Conclusion: A combination of NUS1 and RCN1 accurately distinguished patients from controls, and this combination can be implemented as a screening test for OSCC.