RT Journal Article
SR Electronic
T1 Characterization of Optimal Strain, Frequency and Duration of Mechanical Loading on Skeletal Myotubes' Biological Responses
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 1779
OP 1788
DO 10.21873/invivo.11972
VO 34
IS 4
A1 ATHANASIOS MOUSTOGIANNIS
A1 ANASTASSIOS PHILIPPOU
A1 EVANGELOS ZEVOLIS
A1 ORJONA TASO
A1 ANTONIOS CHATZIGEORGIOU
A1 MICHAEL KOUTSILIERIS
YR 2020
UL http://iv.iiarjournals.org/content/34/4/1779.abstract
AB Background/Aim: Mechanical loading of differentiated myoblasts in vitro may mimic loading patterns of skeletal muscle in vivo. However, it is still uncharacterized the loading conditions that can produce the most effective muscle cells' biological responses, in vitro. This study investigated the effects of different loading protocols on the expression of myogenic regulatory factors, anabolic, atrophy and pro-apoptotic factors in skeletal myotubes. Materials and Methods: C2C12 myoblasts were differentiated and underwent various stretching protocols by altering their elongation, frequency and duration, utilizing an in vitro cell tension system. The loading-induced expression changes of MyoD, Myogenin, MRF4, IGF-1 isoforms, Murf1, Atrogin, Myostatin, Foxo and Fuca were measured by Real Time-PCR. Results: Stretching by 2% elongation at 0.25 Hz for 12 h was overall the most effective in inducing beneficial responses. Conclusion: A low strain, low frequency intermediate duration stretching can most effectively up-regulate myogenic/anabolic factors and down-regulate pro-apoptotic and atrophy genes in myotubes.