RT Journal Article
SR Electronic
T1 Biological Properties of the Aggregated Form of Chitosan Magnetic Nanoparticle
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 1729
OP 1738
DO 10.21873/invivo.11966
VO 34
IS 4
A1 ANGEL DAVID PAULINO-GONZALEZ
A1 HIROSHI SAKAGAMI
A1 KENJIRO BANDOW
A1 YUMIKO KANDA
A1 YUKO NAGASAWA
A1 YASUSHI HIBINO
A1 HIROSHI NAKAJIMA
A1 SATOSHI YOKOSE
A1 OSAMU AMANO
A1 GIICHIROU NAKAYA
A1 YUKARI KOGA-OGAWA
A1 AKIYOSHI SHIROTO
A1 TADAMASA NOBESAWA
A1 DAISUKE UEDA
A1 SACHIE NAKATANI
A1 KENJI KOBATA
A1 YOSUKE IIJIMA
A1 SHINSUKE IFUKU
A1 MASAJI YAMAMOTO
A1 RENE GARCIA-CONTRERAS
YR 2020
UL http://iv.iiarjournals.org/content/34/4/1729.abstract
AB Background/Aim: Chitosan-coated iron oxide nanoparticles (Chi-NP) have gained attention because of their biocompatibility, biodegradability, low toxicity and targetability under magnetic field. In this study, we investigated various biological properties of Chi-NP. Materials and Methods: Chi-NP was prepared by mixing magnetic NP with chitosan FL-80. Particle size was determined by scanning and transmission electron microscopes, cell viability by MTT assay, cell cycle distribution by cell sorter, synergism with anticancer drugs by combination index, PGE2 production in human gingival fibroblast was assayed by ELISA. Results: The synthetic process of Chi-NP from FL-80 and magnetic NP increased the affinity to cells, up to the level attained by nanofibers. Upon contact with the culture medium, Chi-NP instantly formed aggregates and interfered with intracellular uptake. Aggregated Chi-NP did not show cytotoxicity, synergism with anticancer drugs, induce apoptosis (accumulation of subG1 cell population), protect the cells from X-ray-induced damage, nor affected both basal and IL-1β-induced PGE2 production. Conclusion: Chi-NP is biologically inert and shows high affinity to cells, further confirming its superiority as a scaffold for drug delivery.