TY - JOUR T1 - Biological Properties of the Aggregated Form of Chitosan Magnetic Nanoparticle JF - In Vivo JO - In Vivo SP - 1729 LP - 1738 DO - 10.21873/invivo.11966 VL - 34 IS - 4 AU - ANGEL DAVID PAULINO-GONZALEZ AU - HIROSHI SAKAGAMI AU - KENJIRO BANDOW AU - YUMIKO KANDA AU - YUKO NAGASAWA AU - YASUSHI HIBINO AU - HIROSHI NAKAJIMA AU - SATOSHI YOKOSE AU - OSAMU AMANO AU - GIICHIROU NAKAYA AU - YUKARI KOGA-OGAWA AU - AKIYOSHI SHIROTO AU - TADAMASA NOBESAWA AU - DAISUKE UEDA AU - SACHIE NAKATANI AU - KENJI KOBATA AU - YOSUKE IIJIMA AU - SHINSUKE IFUKU AU - MASAJI YAMAMOTO AU - RENE GARCIA-CONTRERAS Y1 - 2020/07/01 UR - http://iv.iiarjournals.org/content/34/4/1729.abstract N2 - Background/Aim: Chitosan-coated iron oxide nanoparticles (Chi-NP) have gained attention because of their biocompatibility, biodegradability, low toxicity and targetability under magnetic field. In this study, we investigated various biological properties of Chi-NP. Materials and Methods: Chi-NP was prepared by mixing magnetic NP with chitosan FL-80. Particle size was determined by scanning and transmission electron microscopes, cell viability by MTT assay, cell cycle distribution by cell sorter, synergism with anticancer drugs by combination index, PGE2 production in human gingival fibroblast was assayed by ELISA. Results: The synthetic process of Chi-NP from FL-80 and magnetic NP increased the affinity to cells, up to the level attained by nanofibers. Upon contact with the culture medium, Chi-NP instantly formed aggregates and interfered with intracellular uptake. Aggregated Chi-NP did not show cytotoxicity, synergism with anticancer drugs, induce apoptosis (accumulation of subG1 cell population), protect the cells from X-ray-induced damage, nor affected both basal and IL-1β-induced PGE2 production. Conclusion: Chi-NP is biologically inert and shows high affinity to cells, further confirming its superiority as a scaffold for drug delivery. ER -