RT Journal Article
SR Electronic
T1 Diagnostic Algorithm in Hirschsprung's Disease: Focus on Immunohistochemistry Markers
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 1355
OP 1359
DO 10.21873/invivo.11913
VO 34
IS 3
A1 PRZEMYSLAW GALAZKA
A1 LUKASZ SZYLBERG
A1 MAGDALENA BODNAR
A1 JAN STYCZYNSKI
A1 ANDRZEJ MARSZALEK
YR 2020
UL http://iv.iiarjournals.org/content/34/3/1355.abstract
AB Background/Aim: Hirschsprung disease (HD) is caused by the congenital absence of ganglion cells in the distal bowel (aganglionosis). Rectal biopsy is considered important for its diagnosis. The aim of this study was to apply immunohistochemical staining using a minimal set of antibodies and develop an algorithm that will assist in the diagnosis of HD. Patients and Methods: Rectal or colonic biopsies were performed in patients with HD (n=26) or patients treated for other bowel diseases (n=34). Immunohistochemical staining was performed for MAP1b, peripherin, S-100, calretinin, NSE, bcl-2 and CD56 proteins. Results: Staining for CD56, S-100, peripherin and calretinin facilitated the identification of ganglion cells. The use of CD56 and S-100 antibodies together resulted in the highest rate of ganglion cell staining intensity (94%). Conclusion: We propose a practical diagnostic algorithm with the application of CD56 and S-100 antibodies that can be used in clinical practice in children suspected of Hirschsprung's disease.