TY - JOUR T1 - Detection of Serum miRNAs Affecting Liver Apoptosis in a Periodontitis Rat Model JF - In Vivo JO - In Vivo SP - 117 LP - 123 DO - 10.21873/invivo.11752 VL - 34 IS - 1 AU - YOSHIO SUGIURA AU - TOSHIKI YONEDA AU - KOHEI FUJIMORI AU - TAKAYUKI MARUYAMA AU - HISATAKA MIYAI AU - TERUMASA KOBAYASHI AU - DAISUKE EKUNI AU - TAKAAKI TOMOFUJI AU - MANABU MORITA Y1 - 2020/01/01 UR - http://iv.iiarjournals.org/content/34/1/117.abstract N2 - Background/Aim: miRNA molecules have been attracting attention as genetic modifiers between organs. We examined the relationship between serum miRNA and targeted liver mRNA profiles in a periodontitis rat model, and the influence of periodontitis on the liver. Materials and Methods: Male Wistar rats (n=16, 8 weeks old) were randomly divided into two groups (8 rats each): control and periodontitis (ligature placement for 4 weeks). Serum miRNA and liver mRNA profiles were compared. Results: Periodontal destruction and hepatocyte apoptosis were induced in the periodontitis group. Microarray analysis indicated that 52 serum miRNAs and 33 liver mRNAs were expressed with a >1.5-fold change (FC) and a >2.0-FC (p<0.05), respectively, between the two groups. From the miRNA target genes, 12 genes equivalented to liver mRNAs with a >2.0-FC, among which, Hyou1, Chac1, and Bloc1s3 have apoptotic functions in our model. miRNAs upstream of these 3 mRNAs are miR-3591, miR-181a-2-3p and miR-6321. Conclusion: miR-3591, miR-181a-2-3p and miR-6321 induced hepatocyte apoptosis in our periodontitis rat model. ER -