@article {SUGIURA117, author = {YOSHIO SUGIURA and TOSHIKI YONEDA and KOHEI FUJIMORI and TAKAYUKI MARUYAMA and HISATAKA MIYAI and TERUMASA KOBAYASHI and DAISUKE EKUNI and TAKAAKI TOMOFUJI and MANABU MORITA}, title = {Detection of Serum miRNAs Affecting Liver Apoptosis in a Periodontitis Rat Model}, volume = {34}, number = {1}, pages = {117--123}, year = {2020}, doi = {10.21873/invivo.11752}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: miRNA molecules have been attracting attention as genetic modifiers between organs. We examined the relationship between serum miRNA and targeted liver mRNA profiles in a periodontitis rat model, and the influence of periodontitis on the liver. Materials and Methods: Male Wistar rats (n=16, 8 weeks old) were randomly divided into two groups (8 rats each): control and periodontitis (ligature placement for 4 weeks). Serum miRNA and liver mRNA profiles were compared. Results: Periodontal destruction and hepatocyte apoptosis were induced in the periodontitis group. Microarray analysis indicated that 52 serum miRNAs and 33 liver mRNAs were expressed with a \>1.5-fold change (FC) and a \>2.0-FC (p\<0.05), respectively, between the two groups. From the miRNA target genes, 12 genes equivalented to liver mRNAs with a \>2.0-FC, among which, Hyou1, Chac1, and Bloc1s3 have apoptotic functions in our model. miRNAs upstream of these 3 mRNAs are miR-3591, miR-181a-2-3p and miR-6321. Conclusion: miR-3591, miR-181a-2-3p and miR-6321 induced hepatocyte apoptosis in our periodontitis rat model.}, issn = {0258-851X}, URL = {https://iv.iiarjournals.org/content/34/1/117}, eprint = {https://iv.iiarjournals.org/content/34/1/117.full.pdf}, journal = {In Vivo} }