RT Journal Article
SR Electronic
T1 Second-generation Probiotics Producing IL-22 Increase Survival of Mice After Total Body Irradiation
JF In Vivo
JO In Vivo
FD International Institute of Anticancer Research
SP 39
OP 50
DO 10.21873/invivo.11743
VO 34
IS 1
A1 ZHANG, XICHEN
A1 FISHER, RENEE
A1 HOU, WEN
A1 SHIELDS, DONNA
A1 EPPERLY, MICHAEL W.
A1 WANG, HONG
A1 WEI, LIANG
A1 LEIBOWITZ, BRIAN J.
A1 YU, JIAN
A1 ALEXANDER, LAURA M.
A1 VAN PIJKEREN, JAN-PETER
A1 WATKINS, SIMON
A1 WIPF, PETER
A1 GREENBERGER, JOEL S.
YR 2020
UL http://iv.iiarjournals.org/content/34/1/39.abstract
AB Background/Aim: Intestinal damage induced by total body irradiation (TBI) reduces leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5)-expressing stem cells, goblet, and Paneth cells, breaching the epithelial lining, and facilitating bacterial translocation, sepsis, and death. Materials and Methods: Survival was measured after TBI in animals that received wild-type or recombinant bacteria producing interleukin-22 (IL-22). Changes in survival due to microbially delivered IL-22 were measured. Lactobacillus reuteri producing IL-22, or Escherichia coli-IL-22 were compared to determine which delivery system is better. Results: C57BL/6 mice receiving IL-22 probiotics at 24 h after 9.25 Gy TBI, demonstrated green fluorescent protein-positive bacteria in the intestine, doubled the number of Lgr5+ intestinal stem cells, and increased 30-day survival. Bacteria were localized to the jejunum, ileum, and colon. Conclusion: Second-generation probiotics appear to be valuable for mitigation of TBI, and radiation protection during therapeutic total abdominal irradiation.