PT  - JOURNAL ARTICLE
AU  - ZHANG, XICHEN
AU  - FISHER, RENEE
AU  - HOU, WEN
AU  - SHIELDS, DONNA
AU  - EPPERLY, MICHAEL W.
AU  - WANG, HONG
AU  - WEI, LIANG
AU  - LEIBOWITZ, BRIAN J.
AU  - YU, JIAN
AU  - ALEXANDER, LAURA M.
AU  - VAN PIJKEREN, JAN-PETER
AU  - WATKINS, SIMON
AU  - WIPF, PETER
AU  - GREENBERGER, JOEL S.
TI  - Second-generation Probiotics Producing IL-22 Increase Survival of Mice After Total Body Irradiation
AID  - 10.21873/invivo.11743
DP  - 2020 Jan 01
TA  - In Vivo
PG  - 39--50
VI  - 34
IP  - 1
4099  - http://iv.iiarjournals.org/content/34/1/39.short
4100  - http://iv.iiarjournals.org/content/34/1/39.full
SO  - In Vivo2020 Jan 01; 34
AB  - Background/Aim: Intestinal damage induced by total body irradiation (TBI) reduces leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5)-expressing stem cells, goblet, and Paneth cells, breaching the epithelial lining, and facilitating bacterial translocation, sepsis, and death. Materials and Methods: Survival was measured after TBI in animals that received wild-type or recombinant bacteria producing interleukin-22 (IL-22). Changes in survival due to microbially delivered IL-22 were measured. Lactobacillus reuteri producing IL-22, or Escherichia coli-IL-22 were compared to determine which delivery system is better. Results: C57BL/6 mice receiving IL-22 probiotics at 24 h after 9.25 Gy TBI, demonstrated green fluorescent protein-positive bacteria in the intestine, doubled the number of Lgr5+ intestinal stem cells, and increased 30-day survival. Bacteria were localized to the jejunum, ileum, and colon. Conclusion: Second-generation probiotics appear to be valuable for mitigation of TBI, and radiation protection during therapeutic total abdominal irradiation.