<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">ZHANG, XICHEN</style></author><author><style face="normal" font="default" size="100%">FISHER, RENEE</style></author><author><style face="normal" font="default" size="100%">HOU, WEN</style></author><author><style face="normal" font="default" size="100%">SHIELDS, DONNA</style></author><author><style face="normal" font="default" size="100%">EPPERLY, MICHAEL W.</style></author><author><style face="normal" font="default" size="100%">WANG, HONG</style></author><author><style face="normal" font="default" size="100%">WEI, LIANG</style></author><author><style face="normal" font="default" size="100%">LEIBOWITZ, BRIAN J.</style></author><author><style face="normal" font="default" size="100%">YU, JIAN</style></author><author><style face="normal" font="default" size="100%">ALEXANDER, LAURA M.</style></author><author><style face="normal" font="default" size="100%">VAN PIJKEREN, JAN-PETER</style></author><author><style face="normal" font="default" size="100%">WATKINS, SIMON</style></author><author><style face="normal" font="default" size="100%">WIPF, PETER</style></author><author><style face="normal" font="default" size="100%">GREENBERGER, JOEL S.</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Second-generation Probiotics Producing IL-22 Increase Survival of Mice After Total Body Irradiation</style></title><secondary-title><style face="normal" font="default" size="100%">In Vivo</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2020-01-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">39-50</style></pages><doi><style  face="normal" font="default" size="100%">10.21873/invivo.11743</style></doi><volume><style face="normal" font="default" size="100%">34</style></volume><issue><style face="normal" font="default" size="100%">1</style></issue><abstract><style  face="normal" font="default" size="100%">Background/Aim: Intestinal damage induced by total body irradiation (TBI) reduces leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5)-expressing stem cells, goblet, and Paneth cells, breaching the epithelial lining, and facilitating bacterial translocation, sepsis, and death. Materials and Methods: Survival was measured after TBI in animals that received wild-type or recombinant bacteria producing interleukin-22 (IL-22). Changes in survival due to microbially delivered IL-22 were measured. Lactobacillus reuteri producing IL-22, or Escherichia coli-IL-22 were compared to determine which delivery system is better. Results: C57BL/6 mice receiving IL-22 probiotics at 24 h after 9.25 Gy TBI, demonstrated green fluorescent protein-positive bacteria in the intestine, doubled the number of Lgr5+ intestinal stem cells, and increased 30-day survival. Bacteria were localized to the jejunum, ileum, and colon. Conclusion: Second-generation probiotics appear to be valuable for mitigation of TBI, and radiation protection during therapeutic total abdominal irradiation.</style></abstract></record></records></xml>