@article {ZHANG39, author = {XICHEN ZHANG and RENEE FISHER and WEN HOU and DONNA SHIELDS and MICHAEL W. EPPERLY and HONG WANG and LIANG WEI and BRIAN J. LEIBOWITZ and JIAN YU and LAURA M. ALEXANDER and JAN-PETER VAN PIJKEREN and SIMON WATKINS and PETER WIPF and JOEL S. GREENBERGER}, title = {Second-generation Probiotics Producing IL-22 Increase Survival of Mice After Total Body Irradiation}, volume = {34}, number = {1}, pages = {39--50}, year = {2020}, doi = {10.21873/invivo.11743}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: Intestinal damage induced by total body irradiation (TBI) reduces leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5)-expressing stem cells, goblet, and Paneth cells, breaching the epithelial lining, and facilitating bacterial translocation, sepsis, and death. Materials and Methods: Survival was measured after TBI in animals that received wild-type or recombinant bacteria producing interleukin-22 (IL-22). Changes in survival due to microbially delivered IL-22 were measured. Lactobacillus reuteri producing IL-22, or Escherichia coli-IL-22 were compared to determine which delivery system is better. Results: C57BL/6 mice receiving IL-22 probiotics at 24 h after 9.25 Gy TBI, demonstrated green fluorescent protein-positive bacteria in the intestine, doubled the number of Lgr5+ intestinal stem cells, and increased 30-day survival. Bacteria were localized to the jejunum, ileum, and colon. Conclusion: Second-generation probiotics appear to be valuable for mitigation of TBI, and radiation protection during therapeutic total abdominal irradiation.}, issn = {0258-851X}, URL = {https://iv.iiarjournals.org/content/34/1/39}, eprint = {https://iv.iiarjournals.org/content/34/1/39.full.pdf}, journal = {In Vivo} }