RT Journal Article SR Electronic T1 Effect of Robinia pseudoacacia Leaf Extract on Interleukin-1β–mediated Tumor Angiogenesis JF In Vivo JO In Vivo FD International Institute of Anticancer Research SP 1901 OP 1910 DO 10.21873/invivo.11684 VO 33 IS 6 A1 HEE SOO KIM A1 JI MIN JANG A1 SO YOON YUN A1 DAN ZHOU A1 YONGWEI PIAO A1 HAE CHAN HA A1 MOON JUNG BACK A1 IN CHUL SHIN A1 DAE KYONG KIM YR 2019 UL http://iv.iiarjournals.org/content/33/6/1901.abstract AB Background/Aim: Interleukin (IL)-1β is a pro-inflammatory cytokine that has recently been established as a stimulator of angiogenesis via regulation of proangiogenic factor expression in the tumor microenvironment. This study aimed to demonstrate the inhibitory effects of Robinia pseudoacacia leaf extract (RP) on IL-1β–mediated tumor angiogenesis. Materials and Methods: Secreted embryonic alkaline phosphatase (SEAP) reporter gene assay, ex vivo and in vitro tube formation assay, western blot, and quantitative PCR were used to analyze the inhibitory effect of RP on IL-1β–mediated angiogenesis. Results: RP inhibited secretion of SEAP, blocked IL-1β signaling, and inhibited IL-1β–mediated angiogenesis in ex vivo and in vitro assays. RP inhibited nuclear translocation of NF-ĸB by suppressing phosphorylation of IL-1β signaling protein kinases and inhibited mRNA expression of IL-1β–induced pro-angiogenic factors including VEGFA, FGF2, ICAM1, CXCL8, and IL6. Conclusion: RP suppressed IL-1β–mediated angiogenesis and, thus, could be a promising agent in anticancer therapy.