The Asn680Ser Variant Negatively Impacted the Ovarian Response to Controlled Ovarian Stimulation in Thai Female Infertility

Representative electrophoretic analysis of PCR–RFLP products and corresponding Sanger sequencing chromatograms for the analyzed polymorphisms. (A) Asn680Ser polymorphism. Agarose gel electrophoresis of restriction fragments and representative sequencing chromatograms showing the Asn/Asn, Asn/Ser, and Ser/Ser genotypes. N indicates the Asn (normal) allele; S indicates the Ser (variant) allele. (B) −29G>A polymorphism. Electrophoretic patterns and sequencing chromatograms illustrating GG, GA, and AA genotypes. G represents the normal allele; A represents the variant allele. (C) −211G>T polymorphism. Representative sequencing chromatograms demonstrating GG and GT genotypes. G denotes the normal allele; T denotes the variant allele. M: DNA fragment size marker (100–1000 bp ladder); PCR–RFLP: polymerase chain reaction–restriction fragment length polymorphism.