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Microarray Analysis of Human Abdominal Aortic Aneurysm With Emphasis on Cardiovascular Genes Revealed Differentially Expressed Genes

SONG LU, LI PING LI, JOHN V. WHITE, XIAOYING ZHANG, IFEYINWA NWANESHIUDU, ADAOBI NWANESHIUDU, NECTARIA NTAOULA, JOHN GAUGHAN, DIMITRI S. MONOS, WAN-LU LIN, CHARALAMBOS C. SOLOMIDES, EMILIA L. OLESZAK and CHRIS D. PLATSOUCAS
In Vivo May 2026, 40 (3) 1327-1347; DOI: https://doi.org/10.21873/invivo.14286

Article Figures & Data

Figures

  • Figure 1.
    Figure 1.

    Volcano plot of gene expression by microarray analysis. Red colored dots represent genes in abdominal aortic aneurysm that were statistically significantly upregulated compared with normal aortas. Blue colored dots represent genes statistically significantly downregulated. Grey colored dots represent genes with a fold-change (FC) of less than 2 (log2<1), or genes with no statistically significant change.

  • Figure 2.
    Figure 2.

    Genes upregulated in abdominal aortic aneurysms (AAA) as compared with normal aortas. Transcript levels were determined and the results were analyzed as described in the Materials and Methods. Statistical significance was determined using Student’s t-test: *p≤0.05 and **p≤0.01. APOD: Apolipoprotein D; APOE: apolipoprotein E; CCL2: C-C motif chemokine ligand 2; CIB1: calcium and integrin binding 1; COL15A1: collagen type XV α 1 chain; COL1A1: collagen type I α 1 chain; COL6A3: collagen type VI α 3 chain; DCN: decorin; ENG: endoglin; H19: H19 imprinted maternally expressed transcript; ICAM1: intercellular adhesion molecule 1; ICAM2: intercellular adhesion molecule 2; MMP9: matrix metallopeptidase 9; PDGFRB: platelet-derived growth factor receptor beta; PLA2G2A: phospholipase A2 group IIA; PLAU: urokinase-type plasminogen activator; PLTP: phospholipid transfer protein; SERPINB6: serpin family B member 6; TIMP3: TIMP metallopeptidase inhibitor 3; VWF: von Willebrand factor.

  • Figure 3.
    Figure 3.

    Genes downregulated in abdominal aortic aneurysms (AAA) as compared with normal aortas. Transcript levels were determined, and the results were analyzed as described in the Materials and Methods. Statistical significance was determined using Student’s t-test: *p≤0.05 and **p≤0.01. ACAA: Acetyl-CoA acyltransferase; ACTN1: actinin α 1; BGN: biglycan; COL11A2: collagen type XI α 2 chain;COL4A4: collagen type IV α 4 chain; GJA1: gap junction protein α 1; ITGA8: integrin subunit α 8; LGALS1: galectin-1; LRP1: low-density lipoprotein receptor-related protein 1; MCAM: melanoma cell adhesion molecule; NPR1: natriuretic peptide receptor 1; PLN: phospholamban; SCNN1B: sodium channel epithelial 1 subunit beta; SERPINE1: serpin family E member 1; SOD3: superoxide dismutase 3.

  • Figure 4.
    Figure 4.

    Quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) confirmation of genes found by microarray analysis to be upregulated. Log2 ratios of gene expression in a patient with abdominal aortic aneurysm (AAA10) to those in control aorta (aorta 1) by microarray analysis and qRT-PCR analysis. APOD: Apolipoprotein D; APOE: apolipoprotein E; CCL2: C-C motif chemokine ligand 2; COL15A1: collagen type XV α 1 chain; COL1A1: collagen type I α 1 chain; COL6A3: collagen type VI α 3 chain; DCN: decorin; H19: H19 imprinted maternally expressed transcript; ICAM2: intercellular adhesion molecule 2; MMP9: matrix metallopeptidase 9; PLA2G2A: phospholipase A2 group IIA; PLAU: urokinase-type plasminogen activator; PLTP: phospholipid transfer protein; TIMP3: TIMP metallopeptidase inhibitor 3; VWF: von Willebrand factor.

  • Figure 5.
    Figure 5.

    Quantitative real time reverse transcriptase-polymerase chain reaction (qRT-PCR) confirmation of genes found by microarray analysis to be downregulated. Log2 ratios of gene expression in a patient with abdominal aortic aneurysm (AAA10) to those in control aorta (aorta 1) by microarray analysis and qRT-PCR analysis. ACTN1: Actinin α 1; BGN: biglycan; GJA1: gap junction protein α 1; LGALS1: galectin-1; LRP1: low-density lipoprotein receptor-related protein 1; MCAM: melanoma cell adhesion molecule; NPR1: natriuretic peptide receptor 1; SOD3: superoxide dismutase 3.

  • Figure 6.
    Figure 6.

    Quantitative real time reverse transcriptase-polymerase chain reaction results of H19 imprinted maternally expressed transcript (H19) and ribosomal protein S9 genes in a patient with abdominal aortic aneurysm (AAA10) and in control aorta (aorta 1). Note the blue horizontal line is the detection threshold. The crossing point of the blue line and amplification curve is the Ct value.

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Microarray Analysis of Human Abdominal Aortic Aneurysm With Emphasis on Cardiovascular Genes Revealed Differentially Expressed Genes
SONG LU, LI PING LI, JOHN V. WHITE, XIAOYING ZHANG, IFEYINWA NWANESHIUDU, ADAOBI NWANESHIUDU, NECTARIA NTAOULA, JOHN GAUGHAN, DIMITRI S. MONOS, WAN-LU LIN, CHARALAMBOS C. SOLOMIDES, EMILIA L. OLESZAK, CHRIS D. PLATSOUCAS
In Vivo May 2026, 40 (3) 1327-1347; DOI: 10.21873/invivo.14286
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