Article Figures & Data
Figures
- Figure 1.
Peripheral blood erythrocytes of mice treated with chromium trioxide. (A) Fluorescent microphotograph (1,000×) using the AO coating method. PCE stain fluorescent orange (i), NCE do not stain (dark shadow) (ii), and MN fluoresces yellow (iii). (B) Kinetics of the MN (mean±S.E./1,000 PCE) observed from 0 to 72 h with the 20 and 25 mg/kg CrO3 treatments. A total of 4,000 PCE were evaluated in each mouse (n=5 mice/group). ^Three and five mice died at 60 and 72 h, respectively. ^Five mice died at 24 h. Statistical significance was determined using two-way repeated measures-ANOVA followed by Tukey’s post-hoc test. Analysis by treatments: *p<0.05 vs. control; +p<0.05 vs. 0 h. Control: Vehicle only; CrO3: chromium trioxide; PCE: polychromatic erythrocytes; NCE: normochromatic erythrocytes; MN: micronuclei; AO: acridine orange; SE: standard error; ANOVA: analysis of variance.
- Figure 2.
Peripheral blood erythrocytes of mice treated with chromium trioxide. (A) Microphotograph (1,000×) using fluorescence in situ hybridization (FISH) with a centromeric DNA probe. PCE stain fluorescent pale red (i), NCE does not stain (white shadow) (ii), MNK− fluorescent yellow (iii), and MNK+ stain fluorescent yellow with a deep red dot (iv). (B) Data show the MN percentages (MNK+ or MNK− divided by the total MN) observed at 0, 12, and 24 h. A total of 4,000 PCE were evaluated in each mouse (n=5 mice/group). Fisher’s exact test: ap<0.0002 vs. CrO3, 0 h; bp<0.0001 vs. control 12 h; cp<0.0001 vs. control, 48 h. Control: Vehicle only; CrO3: chromium trioxide; PCE: polychromatic erythrocytes; NCE: normochromatic erythrocytes; MN: micronuclei; MNK–: micronuclei without centromeric DNA; MNK+: micronuclei with centromeric DNA.
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