Article Figures & Data
Figures
- Figure 1.
Surgical technique to establish primary adrenal neuroblastoma tumors. (A) The mouse was anesthetized, shaved, and the subcostal space was using anatomical landmarks. (B) A subcostal incision was performed. (C-D) The left adrenal gland was visualized. (E) Neuroblastoma cells were injected into the left adrenal gland.
- Figure 2.
Surgical technique to establish extra-adrenal primary neuroblastoma tumors. (A) The mouse was anesthetized, shaved, and intervertebral spaces were visualized using anatomical landmarks. (B) A 1cm skin incision was made above the left 7th rib. (C) The chest wall muscles were retracted, the intercostal muscle was opened, and the chest entered. (D) Once the chest was opened, the left lung deflated, allowing visualization of the vertebral bodies and articulating ribs. (E) Neuroblastoma cells were injected just beneath the pleura between the 5th and 6th rib. (F) The rib was reapproximated with a single suture and air was aspirated after closing the muscle layer, allowing the lung to re-expand. (G) The incision was sutured, and the mouse monitored for complications post-surgery.
- Figure 3.
Primary tumor growth and metastasis in vivo. (A) Combined survival proportion for all experiments with 9464D neuroblastoma cells combined. Adrenal tumors n=22, thorax tumors n=15. (B) Representative image of primary adrenal tumor (outlined) at week 4. (C-D) Representative image of primary thorax tumor (outlined) at week 4. (E) Ultrasound-guided image of primary adrenal tumor (white arrowhead) at week 4. (F) Ultrasound-guided image of thoracic tumor (white arrowhead) at week 4. (G) Primary adrenal tumor metastatic (outlined) to the lung. (H) Primary adrenal tumor metastatic (outlined) to the liver.
- Figure 4.
Quantification of primary tumor luciferase expression. (A) Representative bioluminescent images of mice with adrenal (left) and thoracic (right) primary tumors at indicated time points. (B) Quantification of adrenal primary tumor region of interest (ROI) radiance at indicated time points, n=6. (C) Quantification of thoracic primary tumor region of interest (ROI) radiance at indicated time points, n=5.
- Figure 5.
Representative histology of primary tumors ex vivo. (A-B) Representative H&E section of primary adrenal tumors. (C-D) Representative H&E section of primary thoracic tumors.
