Figure 4. Effects of miglustat, D-PDMP and D-PPMP on the proliferation and mortality rate of MC3T3-E1 cells. (A, B) After exposure to 5, 10, 20, and 50 μM miglustat for 4 days, the cells were plated at 1.0×105 cells per 60-mm dish and cultured for 2 days. After staining the cells with trypan blue, the number of live (A) and dead (B) cells was counted using a hemacytometer. (C, D) After exposure to 5, 10 and 20 μM D-PDMP for 3 days, the cells were plated at 1.0×105 cells per 60-mm dish and cultured for 2 days. After staining the cells with trypan blue, the number of live (C) and dead (D) cells was counted using a hemacytometer. (E, F) After exposure to 0.1, 0.5, 1.0 and 5.0 μM D-PPMP for 3 days, the cells were plated at 1.0×105 cells per 60-mm dish and cultured for 2 days. After staining the cells with trypan blue, the number of live (E) and dead (F) cells was counted using a hemacytometer. Data are expressed as mean±standard deviation. *p<0.05 and **p<0.01.